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Purified DNA was denatured by heating and MeDIP and hMeDIP were conducted with 5mC or 5hmC antibodies as we previously described 64

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TGP sybodies enabled affinity purification of membrane proteins As part of the GFP toolkit, single-chain camelid antibodies (nanobodies) 67,68 and designed ankyrin repeat proteins (DARPins) 69 are available for affinity chromatography, contributing to the purification of several high-profile membrane proteins for structure determination 70,71,72,73,74,75

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