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The cells were collected 48 h post-transfection, washed twice with PBS and lysed in 1 ml of HEPES buffer (20 mM HEPES-KOH (pH 7.9), 300 mM KCl, 0.2 % NP-40, 15 % glycerol, 1 mM DTT and protease inhibitors, Sigma-Aldrich, P8340) for 20 min

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The improvement of intestinal dysbiosis and hepatic metabolic dysfunction in dextran sulfate sodium-induced colitis mice: effects of curcumin

Chronic alcohol feeding inhibits physiological and molecular parameters of intestinal and renal riboflavin transport