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Furthermore, ADDM Network data reveals a positive association between sagging eye syndrome (SES) and ASD diagnosis as well as persistent racial and ethnic disparities in ASD among low-SES children [164]

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A 20 l mix, containing 8 l of cDNA sample, 10 l of SYBR Green PCR Master Mix (Thermo Fisher Scientific), and 2 l of 10 mM forward primer and reverse primer mixture was used to performed Quantitative RT-PCR with a StepOne Plus Real-time PCR system (Applied Biosystems, Foster City, CA, United States)

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