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Pierce the stopper of the BAC water vial and inject the air

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21, 23 Consider supplementation when the patient is prescribed medications causing folate deficiency, e.g., methotrexate

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Protocol and registration This systematic review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement (Moher et al., 2009) and the guidelines from the Cochrane Handbook for Systematic Reviews of Interventions (Higgins and Green, 2008)