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Cell culture and transfection Human embryonic kidney 293 (HEK-293, CVCL_0045) cells were obtained from the American Type Culture Collection (Manassas, VA, USA) and cultured in minimal essential medium supplemented with 10% fetal bovine serum (FBS

However, there was no difference in the level of serum folate between groups, suggesting that long-term folate status, rather than recent folate intake, may be associated with the risk of AD (162)

Rely on peer-reviewed journals, guideline papers from reproductive medicine societies, and clinicaltrials.gov

The pathway genes with strong constitutive GAP promoters were introduced into the genome of W303-1b via homologous integration at -sites

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