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tumefaciens cultures carrying SUMM2, CRCK3 or MEKK2 at OD 600 = 1.2 were then added into the above dilution series to examine the effect of increasing LET7 protein levels on SUMM2, CRCK3 or MEKK2 expression

References: Julius M, Lang CA, Gleiberman L, Harburg E, DiFranceisco W, Schork A