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Glucose deprivation experiments used glucose-free DMEM (Thermo, 12100-061) containing 10% dialyzed FBS (Gibco, 30067334)

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We have validated that this TYR-catalyzed prodrug activation approach could effectively retain the intracellular drug concentration to resensitize the drug-resistant melanoma cells to chemotherapeutics both in vitro and in vivo

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