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FIGURE 4 Surprisingly, cascades with TMLH, but lacking one or more (active) enzymes (combination C, D, E, F, and G) also showed L-carnitine biosensor signal output, albeit it was significantly lower than that of the complete cascade (combination H)

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LC-MS/MS Analysis With DDA Samples were injected (30 L) onto an Aeris PEPTIDE XB-C18 100 2.1 mm column, with solid core 1.7 m particles (100 ) fitted with a SecurityGuard ULTRA C18-peptide guard column (Phenomenex, Torrance, CA) using a Nexera UHPLC system (Shimadzu, Columbia, MD) with water (A) and ACN (B), both containing 0.1% formic acid, at a flow rate of 0.3 mL/min (40C)

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