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After 20 min at RT, the fixed biofilm was washed three times for 5 min each with 2 mL of PBS, then blocked with 5% normal donkey serum (Jackson ImmunoResearch Laboratories, catalog no

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[9] Wilding, J.P.H., et al

It can help make these thought patterns less distressing and less likely to evoke serious worry or fear and to trigger depression

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