(10)Fmoc-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val- (10) Preparation of Fmoc-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin: (9)Fmoc-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-1050530105010603DMF3DMF2DMFFmoc-Gly-OHDIEATBTU/HOBtHBTU/HOBtBOP/HOBt105010603DMF3DMF2Fmoc-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val- In the Fmoc-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin in step (9), add a decapping reagent and react at 10-50C for 5-30 minutes , dry it with a vacuum pump, re-add the decapping reagent at 10-50 C for 10-60 minutes, drain it, wash it with isopropanol for 3 times, wash it with DMF for 3 times, wash it twice with DMF, add the solution dissolved in DMF Mixture of Fmoc-Gly-OH, DIEA, TBTU/HOBt or HBTU/HOBt or BOP/HOBt, react at 10-50C for 10-60 minutes, dry it with a vacuum pump, wash with isopropanol for 3 times, DMF for 3 times, The DMF that redistilled is washed 2 times, sucks dry, obtains Fmoc-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin resin
I would like to note that Ive also experimented with these healing peptides enough to notice that there is a specific method of injection that seems to work better than others
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Slower injections are less painful
Bacteriostatic water is sterile water with 0.9% benzyl alcohol added as a preservative, which lets a reconstituted vial be re-entered over several days
It makes more sense when you tie it to the gut barrier and immune reactions
