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Moreover, stem-cell-based treatment is still associated with multiple concerns, such as poor cell survival, immune rejection, complex cell culture procedures, decreased regeneration capacity after transplantation, and ethical concerns 17,18

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A 2nd 100 l of 80 % methanol (80 C) was added to the pellet, the sample was vortexed, centrifuged at 13,000 g for 30 min at 4 C, and the 2nd 100 l of supernatant was saved

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