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Primer sequences used were as follows: GAPDH (F: CCTCGTCCCGTAGACAAAATG, R: TGAGGTCAATGAAGGGGTCGT);ANP (F: CCGATAGATCTGCCCTCTTGAA, R: GCTGTTGCAGCCTAGTCCACT);BNP (F: GGAGGCGAGACAAGGGAGAA, R: CCAGCGGTGACAGATAAAGGAA);-MHC (F: ATGAGGAGTAGCTCTTGTGCTACC, R: CCACCTAAAGGGCTGTTGC);All primers were synthesized by RiboBio (Guangzhou, China)

Copper is a vital cofactor for methionine synthase, and its deficiency can impair the enzymes function, disrupting methylation reactions

Trinh, D., Al Halabi, L., Brar, H., Kametani, M

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First, mRNAs of NRF2, as well as of Heme Oxigenase 1 (HO-1) and GCL, two enzymes whose expression is known to be under the control of NRF2, were quantified by qRT-PCR (Figure 5A)