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The level of OGD-induced ferroptosis in ADSCs was assessed according to the viability of cells treated with the ferroptosis inhibitor Ferrostatin-1 (Fer-1) 5 M, autophagy inhibitor 3-Methyladenine (3-MA) 5 mM, apoptosis inhibitor Z-VAD-FMK 10 M, necroptosis inhibitor Necrostatin-1 (Nec-1) 50 M, or ferroptosis activator erastin

coli TransB (DE3) (purchased from the Beijing TransGen Biotech, Beijing, China) and the bacteria were cultured in LB medium supplemented with ampicillin and spectinomycin

Jacques et al, 1996

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