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Thirty micrograms of protein lysate were loaded onto 4-12% Bis-tris polyacrylamide gels and resolved using MOPS-SDS running buffer

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Reaction of melatonin and related indoles with hydroxyl radicals: Epr and spin trapping investigations

Experimental data suggest that this formula provides a strategy for the prevention and treatment of HACE by intervening in the oxidative-inflammatory cascade and energy metabolism disorders through multi-target mechanisms

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However, a primary hurdle is the standardization and reproducibility of NP-derived compounds, given the natural variability in their sources and compositions, which complicates mechanistic validation and clinical batch production (Birer-Williams et al., 2020)