Recovery of specific bands and protein identification Bands representing HaCaT cellular proteins specifically interacting with GST-HPV-16 E7 were excised from the gel, incubated for 20 min in the dark with destaining solutions provided in the SilverQuest TM Silver Staining kit (Invitrogen), and subjected to tryptic proteolysis, using the In-gel Digest kit (Millipore Corporation, Billerica, MA) as described [39]
10.3390/ijms13033145 Int
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Usually resolves within the first few weeks
