Ethics declarations Ethics approval and consent to participate All animal experiments were complied with the ARRIVE guidelines and performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals
Adv Ther 37:31123128 Colque-Caro LA, Acua F, Aguirre LS, Avellaneda-Cceres A, Barbeito CG, Signorini M, Moore DP, Micheloud JF (2023) Characterization of lesions of nutritional congenital goitre in cattle
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Stable complex formation was checked by SDS-PAGE and the complex was separated from unreacted SnCE1 and SUMO1-PA by performing SEC on an equilibrated HiLoad 16/600 Superdex 75 pg column (Cytiva Germany GmbH, Freiburg DE)
In vitro hypoxia and nutrient deprivation model Cells were cultured to approximately 80% confluence, then the medium was replaced with EBSS containing 200 M cobalt chloride for the indicated times [55]
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