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After washing and permeabilizing, 100 L click reaction solution was added to each well, followed by incubation at room temperature in the dark for 30 min

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Finally, there are also data showing that even though serum IGF-I is acutely increased following prednisolone, this is not the case in lymph-like tissue fluid (107), suggesting that glucocorticoids exert distinct, compartment-specific effects on IGF-I action

Brief exposure to room temperature is usually fine, but extended warm-storage shortens shelf life

It also helps to regenerate other antioxidants, such as Vitamins C & E