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For treatment, 0.33 nmol/g (total protein) of uridine was added to three independent replicates of treatment group and incubated for 10 min at 25 C, while DMSO was added to the other 3 replicates as a control

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doi: 10.3390/antiox10091491 118 HermelM.SweeneyM.NiY.-M.BonakdarR.TriffonD.SuharC.et al

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45 The second step is an irreversible rate-limiting reaction catalyzed by branched-chain -keto acid dehydrogenase (BCKDH), which is phosphorylated and inactivated by the specific kinase BCKDH kinase (BCKDK) and dephosphorylated and activated by Protein phosphatase 1 K (PPM1K)