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How Serial Dilution Works Reconstitute the peptide at a high concentration (e.g., 2 mg/mL) Take an aliquot and dilute 1:1 with bacteriostatic water 1 mg/mL Take an aliquot from the 1 mg/mL solution and dilute 1:1 0.5 mg/mL Continue as needed for the research protocol Important : Each dilution step must use sterile equipment and fresh bacteriostatic water

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