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[DOI] [PubMed] [Google Scholar] 473.Tamang M.K., Ali A., Pertile R.N., Cui X., Alexander S., Nitert M.D., Palmieri C., Eyles D

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The reaction mixture (1 ml) containing Lockes buffer (pH 7.4), 0.2 ml homogenate and 10 l of DCFH-DA (5 mM) was incubated for 45 min at room temperature and the fluorescence intensity of DCF was recorded by excitation at 485 nm and emission at 535 nm using a Synergy TM 2 Multi-function Microplate Reader (Bio-Tek Instruments Inc., Winooski, Vermont, USA)

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