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Metabolic analysis Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured with a 24-well XF Extracellular Flux Analyzer (Agilent Technologies) and Seahorse Wave Controller Software (Agilent Technologies), according to the manufacturers instructions

Reactive oxygen species signalling in plant stress responses

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Pre-treatment of the PBMCs with antioxidants, tiron, L -histidine, and D -mannitol, reduced the observed number of lesions to the same level as the untreated sample, implicating superoxide, singlet oxygen, and hydroxyl radicals, respectively, in lesion formation

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