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After being washed in PBS, the slides were stained with hematoxylin and eosin, mounted with Canada balsam (03984, Sigma-Aldrich), and observed with an epifluorescence microscope (IX71, Olympus, Tokyo, Japan) equipped with a digital imaging system (DP71, Olympus)

This aptitude for oxidative stress modulation is important in conditions marked by persistent inflammation and immune system anomalies [3, 17]

Upon observing mild adverse events, protocols often call for dose reduction, temporary suspension, or discontinuation depending on severity and causality

Figure 3 shows that the delivery of IGF-1 protein to each layer of cartilage plays a therapeutic role