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24 h post transfection, cells were fixed with 4% PFA for 10 min at RT, washed twice with PBS and permeabilized using a 0.1% NP-40 solution in PBS for 10 min at RT

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TB-500 reconstitution follows a similar protocol: 12 mL of bacteriostatic water per 5 mg vial

doi: 10.1161/CIRCRESAHA.121.318093 42 KimYWangWOklaMKangIMoreauRChungS

Mast Cell Activation Syndrome (MCAS): Mast cells are highly reactive to environmental toxins and oxidative stress