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The spheres were washed twice and stained with 1:500 PI solution (Bio-Sciences) supplemented with 250 g/mL RNAse A (Qiagen) and Hoechst nuclear counterstain (2 g/mL) (Bio-Sciences) for 30 min protected from light, at room temperature
Stage 2: Full liquids (Days 314)
8 weeks 2 vials 8 full weekly maintenance doses
Inject slowly down the inside wall of the peptide vial
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Typical Dosing Ranges Research protocols and practitioner-reported experience consistently place MOTS-C dosing in the 5 to 10 mg range per injection , administered subcutaneously
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