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To investigate the role of IGF-1 in the regulatory effects of miR-221 on MAPK and PI3K/AKT pathways, we conducted western blot experiments to detect changes in these pathways in DPCs/DSCs following treatment with miR-221, miR-221+IGF-1, or IGF-1 alone

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doi: 10.3390/ijms23094992 22 ZhuHYWangHJLiuP

For immunoprecipitation, lysate (100 g of protein) was brought to a total volume of 1 ml with lysis buffer containing 0.5 g antibody and 20 l protein A/G PLUSagarose beads (Santa Cruz Biotechnology, CA)

10.1016/j.joca.2013.01.014 Osteoarthr