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To enable intracellular staining, cells were fixed with Pierce 1.6% formaldehyde (Thermo Fisher Scientific) in Maxpar PBS for 10 min at RT and washed twice with Maxpar Cell Staining Buffer

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Certain parts of the body, such as the armpits and bikini area, may have darker hair that will respond well to treatment

Aconitase is highly sensitive to inactivation by superoxide because of its labile iron atom and its proximity to the superoxide-generating electron transport chain

For severe deficiency, the AAFP recommends loading doses typically daily or every other day for one to two weeks followed by monthly maintenance injections