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In the current study, CBD augmented an increase in HIF-1 levels recorded 24 h after exposure to hypoxia, likely by chelating transition metal ions 22 that inhibit the enzyme responsible for HIF-1 degradation 46,47

FT-IR spectroscopy analysis was carried out with a Bruker Tensor 27 Spectrometer using in the range of 4000 to 400 cm 1 at room temperature

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Methods Enzymol 186:464478 Li W, Li Z, Zhou D, Zhang X, Yan J, Huang G (2019) Maternal folic acid deficiency stimulates neural cell apoptosis via miR-34a associated with Bcl-2 in the rat foetal brain

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If your calculated volume falls below 0.03 mL, you are working with such a tiny amount that even mechanical precision reaches its limits