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This timeline reflects two independent degradation mechanisms: peptide bond hydrolysis and bacterial contamination

Sterile water, lacking a preservative, is better suited for single-use scenarios or procedures where the absence of additives is preferred, such as in sensitive patients or irrigation tasks

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However, significant color changes, cloudiness, or particles indicate degradation

Researchers should not treat them as substitutes for each other based solely on their shared appearance in tissue-repair literature

Maintain refrigeration only between 36-46F (2-8C)