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The blocks were sectioned at 6 m thickness using a Leica microtome (Model RM 2255)

Furthermore, in relation to the oxidative stress as a co-factor of defective oocyte maturation, an appropriate intake of proteins, antioxidants and methyl-donor supplements (1-Carbon Cycle) may decrease the bioavailability of toxic oxidants resulting in the protection of oocyte maturation

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1), suggesting that in such culture conditions, the TSS pathway is active, and CBS contributes to the regulation of cysteine biosynthesis

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