Protocol Gellert-Mortimer et al [2] diluted the 27.6% Nycodenz stock solution with a Hams F10 medium supplemented with 1mM calcium lactate, 20 mM NaHCO3, 5 mM KHCO3 and 0.5 mM MgSO4, to produce the gradient solutions
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Yamazaki N, Yamanaka Y, Hashimoto Y, Shinohara Y, Shima A, Terada H: Structural features of the gene encoding human muscle type carnitine palmitoyltransferase I