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Then cells were fixating by 4% paraformaldehyde, blocking by 1% bovine serum albumin (BSA), incubating with the primary antibody (Calreticulin Rabbit Monoclonal Antibody) at 4 C overnight, staining with the secondary antibody (Goat Anti-Rabbit IgG (Alexa Fluor@488)) for 1 h and DAPI for 10 min at room temperature

The most important of the inflammation-promoting pathways activated by TNF in this case are p38 MAP kinase along with NF-kB

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This comparison highlights that although Spirulina is a valuable functional ingredient for managing diabetes, incorporating or studying red seaweed peptides may offer additional therapeutic potential, suggesting a promising avenue for combinatorial or synergistic approaches in future research