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The pore-forming claudin (CLDN)-2 and barrier forming CLDN-12 seem to be crucial in the Ca 2+ transport through tight junction proteins (17)

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Samples in Laemmli buffer were boiled at 95 C for five min and applied to a 15% SDS gel, separated by SDS-PAGE, and stained with coomassie

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For example, a 36-week trial involving children aged 3-12 years revealed caregiver-rated enhancements, especially on measures like the Aberrant Behavior Checklist (ABC)