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Anal Biochem 59:583598

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Side-effect risk increases with improper dosing, contaminated material, or non-sterile injection technique

ROS and lipid peroxidation assay Day 12 cultured SCD erythroblasts under different treatments were incubated with 5 M 2-7-dichlorodihydrofluorescein diacetate (H2DCFDA) (#D6883, Sigma) for 30 min at 37 C, washed with PBS, and analyzed on a BD LSR-II flow cytometer for ROS level

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Mitochondria freshly isolated from the livers of mice treated with SR4 showed an increase in basal respiration (state 2) of 19.0% compared with control