The membrane was incubated with rabbit anti-Glutathione Peroxidase 4/GPX4 antigen affinity purified polyclonal antibody (Catalog # A02059-1) at 0.5 g/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT
These data are protected by a confidentiality agreement with the study sponsor, Zambon S.p.A., Italy, due to their ethically and commercially sensitive nature
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The procedure was repeated with the reaction mixture containing solution of enzyme (glutathione transferase (GTs))
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