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Separations were performed on a ZORBAX SB-C18 column (150 mm 4.6 mm i.d.) with 5-m particle size (Agilent Technologies, USA) preceded by a 5-m ZORBAX SB-C18 (12.5 mm 4.6 mm i.d.) analytical guard column, with a mobile phase of methanol and 0.2% acetic acid (25:75, v/v) at a flow rate of 0.8 mL/min

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