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Remarkably, in addition to their cancer cell elimination abilities, the introduced macrophages polarized resident M2 tumor-associated macrophages into a M1-like phenotype in a paracrine-like manner

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A total of 700 l of thiol-specific biotinylation reaction contained 70 g of total RNA, 70 l of 10 Biotinylation Buffer (100 mM Tris-Cl pH 7.5 and 10 mM EDTA), 140 l of biotin-HPDP (in 1 mg/ml DMF), and 70 l of DMF