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PKG: Protein kinase-G POD: Peroxidase PLP: Pyridoxal 5-phosphate ROS: Reactive oxygen species SAG: Senescence-activated promoter SAM: S-adenosyl-methionine SOD: Superoxide dismutase SSA: Succinic semi-aldehyde SSADH: Succinic semi-aldehyde dehydrogenase SSR: Succinic semi-aldehyde reductase References Abd Elbar OH, Elkelish A, Niedbaa G et al (2021) Protective effect of -aminobutyric acid against chilling stress during reproductive stage in tomato plants through modulation of sugar metabolism, chloroplast integrity, and antioxidative defense systems

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The lysate was cleared by centrifugation and the protein was purified by Ni-His-affinity chromatography in batch mode

Insert the needle into the BAC water vial (or peptide vial) and push the air in before drawing liquid