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Sulforaphane supplementation has been shown to increase hepatic and blood glutathione levels in human studies (Fahey et al., Proc Natl Acad Sci, 1997

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For F-actin and cellular membrane costaining, cells were incubated with deep red actin tracking stain dye (Thermo, A57245) and CellMask green plasma membrane stain dye (Thermo, C37608) at room temperature in the dark for 30 min, washed with 1 PBS and mounted with DAPI-free mounting medium (Beyotime, P0126)

In a study, it was found that GSPE alleviated symptoms of severe liver problems in rats, including liver enlargement, inflammation, and fat buildups

Furthermore, another large twin-based study produced inconclusive results (73)

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