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Investigating the shared genetic architecture between multiple sclerosis and inflammatory bowel diseases

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Eluate incubated with TEV protease was dialysed against 25 mM Tris (pH 7.5), 150 mM NaCl and 1 mM DTT overnight at 4 C, then incubated with Ni-NTA resin for 1 h to remove the His-tagged protease

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