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Cell pellets were carefully thawed on ice and cells resuspended in 7 mL PBS supplemented with 4 L NP-40 (Thermo Scientific) and 100 L cOmplete protease inhibitor cocktail (Sigma-Aldrich), 1 L DNase I (10 mg/mL), lysozyme (Roth), and 800 L glycerol (Roth)

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10.1016/0092-8674(94)90544-4 Cell 51 LimJ