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In all cases, using equal volumes of supernatants when detecting optical density (OD) is key for accurate comparison

Cell Microbiol (2015) 17:13145.10.1111/cmi.12343 49 ImaiYKubaKNeelyGGYaghubian-MalhamiRPerkmannTVan LooGet alIdentification of oxidative stress and toll-like receptor 4 signaling as a key pathway of acute lung injury

Importantly, cell viability was not affected, meaning the compound did not simply kill fat cells but changed their metabolic behavior

In summary, additional research is needed to understand the intricate molecular mechanisms of p53 in regulating senescence in order to optimize the efficacy and safety for clinical applications

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