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To gain additional insights into the mechanism of inhibition, we conducted a series of structural studies

It helps reduce the chronic low-level inflammation that quietly underlies conditions like acne or rosacea

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Treated cells were fixed with 4% paraformaldehyde for 30 min at room temperature, washed with PBS and permeabilized by treatment with 0.2% Triton X-100 and 5% BSA in PBS for 90 min at room temperature, and incubated in the presence of Nrf2 antibody (1:200) at 4 C