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In addition, collagen expression was measured with a Sirius Red Total Collagen Detection Kit (Chondex, #9062, Redmond, WA, United States), according to the manufacturers instructions, using 1 mg of tissue per mouse

The primary role of antioxidant is to remove free radicals that trigger cell damage from the environment within cells by acting as free radical scavengers or quenchers of singlet oxygen

Higher concentrations aren't necessarily more effective and can increase scalp irritation

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Minard, Raja Muthupillai, Shaji Chacko, Jean W