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The pellet was washed by 5 mL PBS, centrifuged again, and transferred into a microcentrifuge tube (Eppendorf)

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The medication was applied either once, at 1 min after defect creation as an abdominal bath (1 mL /rat) to perceive the initial post-injury course, blood vessel presentation (assessed by USB microscope camera, the blood vessels presentation augmentation/reduction to perceive subsequent course leading to the adhesion formation)

These effects are temporary and typically resolve quickly