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Once the binding interaction finish, the additional buffers are utilized to wash support and remove non-bound components in the samples

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Membranes were blocked in blocking solution (5% Bovine Serum Albumin, 0.01 M TBS, 0.1% Tween-20) at room temperature for 2 hours, and incubated with primary antibodies (DMP1, 1:1000, Abcam