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Cell lines and culture All cells were cultured in DME/F12 (50:50) mixed medium supplemented with 10% fetal calf serum, 2 mM glutamine, 1 mM pyruvate, 100 U ml 1 penicillin and 100 g ml 1 streptomycin at 37 C in a humidified incubator under 5% CO 2

Mild, transient effects during the first two weeks typically do not require intervention beyond patience

B12 injections (hydroxocobalamin) are available on NHS prescription for treating confirmed vitamin B12 deficiency

They are simply volume markings

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