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Bulky side-chain substituents destabilize this arrangement due to steric crowding, so this beta-sheet conformation is usually limited to peptides having a large amount of glycine and alanine
Antibodies of NLRP3 and ASC were obtained from Cell Signaling Technology (Danvers, MA, USA), antibodies of caspase-1 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Santa Cruz Biotechnology, Inc
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GI, gastrointestinal
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