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Once reconstituted with a sterile laboratory solvent, store at 28C and avoid repeated freeze/thaw cycles to maintain compound integrity
As indicated in the Functions of S-Adenosylmethionine (SAM or AdoMet) section below, SAM is the required methyl donor for numerous methylation reactions, including those responsible for epigenetic regulation
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Inhibition of ATM kinase activity in HFM in vitro reduces cell viability To investigate if ATM activity influenced melanocyte survival under sustained elevated ROS conditions in culture we pre-treated cells with the ATM-specific kinase inhibitor KU60019 (which abrogates the kinase function of the protein and prevents downstream phosphorylation of ATM targets 65 ) and incubated these cells with an increased concentration of 250 M H 2 O 2 (to negate the effects of rapid H 2 O 2 deactivation in vitro ensuring sustained elevated ROS during longer treatment times) and 100 M menadione (a naphthoquinone that can generate ROS through redox cycling 66 ) to raise ROS levels, for short (1 h) and long (24 h) durations then measured cell viability
