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Multiple factors play a role in the pathogenesis of ALS, including genetic mutations, oxidative stress, and inflammation [157]

On day 11, cells were washed twice with ice-cold PBS and cell pellets were snap frozen

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Protein concentration was measured by the Bradford method and showed comparable values for all samples with no significant differences before and after treatment (P= 0.943 in a two-tailed paired t-test)

Preliminary findings suggested that interventions in the intestines might significantly weaken these muscular responses

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