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The rate of the activity of SOD was measured the absorption spectrum about formazan form of WST-1 (2-(4-lodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolum, monosodium salt), highly water-soluble tetrazolium salt, that generate a water-soluble formazan dye with reduction of a superoxide anion

All animals were housed in a specific pathogen-free environment in positive pressure rooms at a constant temperature of 22 2 C and relative humidity of 5070% with a standard 12/12-h day-night cycle and fed a standard rodent diet and water ad libitum

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